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pcagspcas9 gfp u6 grna plasmid backbone vector  (Addgene inc)


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    Structured Review

    Addgene inc pcagspcas9 gfp u6 grna plasmid backbone vector
    Pcagspcas9 Gfp U6 Grna Plasmid Backbone Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+backbone+pcag+spcas9+gfp+u6+grna/pCAG-SpCas9-GFP-U6-gRNA+(Plasmid+%2379144)/pm37108564-361-23-27
    Average 94 stars, based on 30 article reviews
    pcagspcas9 gfp u6 grna plasmid backbone vector - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Sequencing:

    Article Title: Store-operated calcium entry is reduced in spastin-linked hereditary spastic paraplegia.
    Article Snippet: .. To generate SPAST KO cells, sgRNAs were designed using the CRISPOR online tool (http://crispor.tefor.net/).35 The Oligonucleotides containing sgRNAs (spacer sequence is depicted in bold) (5′-CACCGgaatagctgttatagttac-3′; 5′-AAACgtaactataacagctattcC-3′ both Sigma-Aldrich) were cloned into the vector backbone pCAG-SpCas9-GFP-U6-gRNA (Addgene #79144) using an adapted version of the protocol developed by the Zhang Lab (https://www. addgene.org/crispr/zhang/). .. Briefly, the vector backbone was digested using BbsI-HF (NEB), CutSmart Buffer (NEB) and dephosphorylated with Calf Intestinal Alkaline Phosphatase (CIP, NEB).

    Article Title: Store-operated calcium entry is reduced in spastin-linked hereditary spastic paraplegia
    Article Snippet: .. The Oligonucleotides containing sgRNAs (spacer sequence is depicted in bold) (5′-CACCG gaatagctgttatagttac -3′; 5′-AAAC gtaactataacagctattc C - 3′ both Sigma-Aldrich) were cloned into the vector backbone pCAG-SpCas9-GFP-U6-gRNA (Addgene #79144) using an adapted version of the protocol developed by the Zhang Lab ( https://www.addgene.org/crispr/zhang/ ). .. Briefly, the vector backbone was digested using BbsI-HF (NEB), CutSmart Buffer (NEB) and dephosphorylated with Calf Intestinal Alkaline Phosphatase (CIP, NEB).

    Clone Assay:

    Article Title: Store-operated calcium entry is reduced in spastin-linked hereditary spastic paraplegia.
    Article Snippet: .. To generate SPAST KO cells, sgRNAs were designed using the CRISPOR online tool (http://crispor.tefor.net/).35 The Oligonucleotides containing sgRNAs (spacer sequence is depicted in bold) (5′-CACCGgaatagctgttatagttac-3′; 5′-AAACgtaactataacagctattcC-3′ both Sigma-Aldrich) were cloned into the vector backbone pCAG-SpCas9-GFP-U6-gRNA (Addgene #79144) using an adapted version of the protocol developed by the Zhang Lab (https://www. addgene.org/crispr/zhang/). .. Briefly, the vector backbone was digested using BbsI-HF (NEB), CutSmart Buffer (NEB) and dephosphorylated with Calf Intestinal Alkaline Phosphatase (CIP, NEB).

    Article Title: Store-operated calcium entry is reduced in spastin-linked hereditary spastic paraplegia
    Article Snippet: .. The Oligonucleotides containing sgRNAs (spacer sequence is depicted in bold) (5′-CACCG gaatagctgttatagttac -3′; 5′-AAAC gtaactataacagctattc C - 3′ both Sigma-Aldrich) were cloned into the vector backbone pCAG-SpCas9-GFP-U6-gRNA (Addgene #79144) using an adapted version of the protocol developed by the Zhang Lab ( https://www.addgene.org/crispr/zhang/ ). .. Briefly, the vector backbone was digested using BbsI-HF (NEB), CutSmart Buffer (NEB) and dephosphorylated with Calf Intestinal Alkaline Phosphatase (CIP, NEB).



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